mat2a inhibitor screening assay kit  (BPS Bioscience)

Journal: Cell Reports Medicine

Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma

doi: 10.1016/j.xcrm.2025.101977

Figure Lengend Snippet: Reversing CD8A + T cell deficiency by methionine metabolism intervention enhancing immunotherapy response in MTAP-deleted osteosarcoma (A) Flowchart depicting methionine restriction diet combined with immune checkpoint therapy. (B and C) In vivo orthotopic tibia models of DuNN shMtap in C3H mice, depicting tumor growth curves (mean ± SD) and tumor weights at experimental endpoints, treated by methionine restriction diet combined with immune checkpoint therapy. ND n = 8; ICT n = 8; MR n = 8; Combined n = 8. Statistical analyses were performed using two-tailed Student’s t tests. (D) Metabolomics of the intestines in DuNN shMtap tumor-bearing C3H mice revealed downregulation of methionine-related metabolites following dietary methionine restriction. (E) Pathological characteristics and immunohistochemistry (CD8A) of tumor tissues from DuNN shMtap in C3H mice, treated by methionine restriction diet combined with immune checkpoint therapy. Arrows indicate T cells. Scale bar, 100 μm (F) Flowchart depicting MAT2A inhibition combined with immune checkpoint therapy. (G and H) In vivo orthotopic tibia models of DuNN shMtap in C3H mice, depicting tumor growth curves (mean ± SD) and survival curve at experimental endpoints, treated by MAT2A inhibition combined with immune checkpoint therapy. ND n = 6; ICT n = 6; MR n = 6; Combined n = 6. Statistical analyses were performed using two-tailed Student’s t tests. (I) Pathological characteristics and immunohistochemistry (CD8A, CD4, and FOXP3) of tumor tissues from DuNN shMtap in C3H mice, treated by MAT2A inhibition combined with immune checkpoint therapy. Arrows indicate T cells. Scale bar, 100 μm.

Article Snippet: MAT2A inhibitor screening assay kit , BPS bioscience , Cat#71402.

Techniques: In Vivo, Two Tailed Test, Immunohistochemistry, Inhibition

Journal: Cell Reports Medicine

Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma

doi: 10.1016/j.xcrm.2025.101977

Figure Lengend Snippet: MAT2A inhibition triggering IKZF1-mediated PD-L1 expression in MTAP-deleted osteosarcoma (A, B, and C) Integrated analysis of transcription factors associated with PD-L1 expression in SGH-OS and TARGET-OS and genes upregulated by treating with MAT2A inhibitor rather than immune checkpoint therapy. Spearman correlation tests were performed, and data with p values < 0.05 were considered statistically significant. (D and E) Protein expression of MAT2A and PD-L1 in MTAP-deleted OS cells (143B) treated with MAT2A inhibitor PF-9366, and protein expression of IKZF1, MAT2A, and PD-L1 in 143B cells co-treated with siIKZF1, assessed by western blotting. (F) IKZF1 RNA expression in wild-type (WT) ( n = 36) and MTAP-deleted (DEL) ( n = 14) cases in SGH-OS cohort. Statistical analyses were performed using two-tailed Student’s t tests. (G) Transcriptomic pathway analysis of cells by treating with PF-9366, using GSEA analysis ( p value < 0.05) on Hallmark gene sets. ES refers to enrichment score, and NES refers to normalized enrichment score. (H and I) Survival curve of TARGET-OS and TCGA-SARC related with IKZF1 expression. Statistical significance was calculated using the log rank test. (J) Summary schematic illustrating the mechanism of methionine intervention enhancing immune checkpoint therapy in MTAP-deleted osteosarcoma (MTAP-del OS).

Article Snippet: MAT2A inhibitor screening assay kit , BPS bioscience , Cat#71402.

Techniques: Inhibition, Expressing, Western Blot, RNA Expression, Two Tailed Test

Journal: Cell Reports Medicine

Article Title: Methionine intervention induces PD-L1 expression to enhance the immune checkpoint therapy response in MTAP-deleted osteosarcoma

doi: 10.1016/j.xcrm.2025.101977

Figure Lengend Snippet:

Article Snippet: MAT2A inhibitor screening assay kit , BPS bioscience , Cat#71402.

Techniques: Control, Recombinant, Saline, Red Blood Cell Lysis, Lysis, Staining, Screening Assay, Transfection, Plasmid Preparation, In Vitro, CCK-8 Assay, Cell Isolation, Gene Expression, Sequencing, Methylation, In Vivo, Software, Microscopy, Refractive Index, Mass Spectrometry